The large fragment patterns (LRFP) and linear
inclusion body profiles of eight tick isolates of spirochete sensu lato were
investigated with pulsed‐field gel ionophoresis (PFGE).
The full cell lysate was examined with atomic number
11 dodecyl sulfate‐polyacrylamide gel ionophoresis (SDS‐PAGE). The MluI LRFP
differentiates 2 species of spirochete sensu lato (Borrelia burgdorferi sensu
stricto and spirochete garinii).
LRFP could be a appropriate technique to demonstrate
genetic hetero‐ or homogeneity of isolates inside one species while not
consequent crossing utilizing various probes. totally different strains with
similar or identical LRFP may be more discriminated by inclusion body profile
analysis. Our results show that every strain analyzed had a unique inclusion
body profile.
Thus the linear inclusion body profile features a
potential application as a strain writing procedure. SDS‐PAGE of whole‐cell
lysate supports the findings of similarity inside the B. burgdorferi sensu
stricto species and also the unsimilarity inside the B. garinii species.
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