Sunday, March 3, 2019

Use of a microorganism Antimicrobial Resistance factor Microarray for the Identification of Resistant coccus aureus



 As diagnostic investigation activities area unit to work out measures required to manage antimicrobial resistance (AMR), new and speedy laboratory strategies area unit necessary to facilitate this vital effort. deoxyribonucleic acid microarray technology permits the detection of an oversized variety of genes in a very single reaction.

This technology is easy, specific and high‐throughput. we've developed a microorganism antimicrobial resistance sequence deoxyribonucleic acid microarray which will permit speedy antimicrobial resistance sequence screening for all Gram‐positive and Gram‐negative microorganism.


An example microarray was designed employing a 70‐mer based mostly oligonucleotide set targeting AMR genes of Gram‐negative and Gram‐positive microorganism. within the gift version, the microarray consists of 182 oligonucleotides equivalent to 166 totally different noninheritable AMR sequence targets, covering most of the resistance genes found in each Gram‐negative and ‐positive microorganism.

A take a look at study was performed on a set of staph aureus isolates from milk samples from dairy farm farms in Québec, Canada. The dependability of the hybridizations made up our minds, and therefore the microarray results were compared with those obtained by makeup resistance tests (either MIC or Kirby‐Bauer).

The microarray genotyping incontestible a correlation between antibiotic drug, antibacterial and E-Mycin resistance phenotypes with the corresponding noninheritable resistance genes. The hybridizations showed that the thirty eight antimicrobial resistant S. aureus isolates possessed a minimum of one AMR sequence.

To Learn More: Join us in the Discussion: 8th European Clinical Microbiology and Immunology Congress on June 12-13, 2019, Edinburgh, Scotland



Contact: Erika Madison
Office Phone: 44 203 769 1755 [Mention Helen/ Erika Madison]
LinkedIn: Erika Madison
Twitter: @MicrobioEvents

No comments:

Post a Comment